Immunocytochemical localization of gentamicin in the rabbit retina following intravitreal injection
C. A. Tabatabay, L. H. Young, D. J. D'Amico and K. R. Kenyon
Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston 02114.
The localization of gentamicin in the retina after a single intravitreal
injection in the rabbit eye was examined by indirect immunofluorescent
staining with goat antigentamicin antiserum. Eight hours after the
injection of 400 micrograms of gentamicin, staining was observed in the
ganglion cell layer, the inner plexiform layer, the inner nuclear layer,
and the photoreceptors. By 12 hours, the staining was also observed in the
retinal pigment epithelium. Howeever, by 24 hours the staining was
predominantly found in the retinal pigment epithelium and choriocapillaris,
and only occasional staining was seen scattered in the neurosensory retina.
At 36 to 48 hours, the labeling was confined to the retinal pigment
epithelium and choriocapillaris. Electron microscopy confirmed the
cytoplasmic localization of gentamicin in the retina.
