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Mark W. Johnson, MD; Karl R. Olsen, MD; Eleut Hernandez; W. David Irvine, MD; Robert N. Johnson, MD

Arch Ophthalmol. 1990;108(2):259-263.

Abstract
• We studied the dose-dependent retinal toxicity of the available commercial preparation of human recombinant tissue plasminogen activator in the normal rabbit eye. Tissue plasminogen activator was injected into the midvitreous cavity of albino rabbits in doses (per 100 µL) of 25, 50, 75, and 100 µg. Control eyes received 100 µL of balanced salt solution or tissue plasminogen activator vehicle. No evidence of a retinal toxic reaction was seen in eyes receiving 25 µg of tissue plasminogen activator. One of four eyes injected with 50 µg showed loss of photoreceptor cells by light microscopy. Severe retinal damage was seen by ophthalmoscopy, electroretinography, and light microscopy in three of four eyes receiving 75 µg of tissue plasminogen activator and in all eyes treated with 100 µg of tissue plasminogen activator or equivalent vehicle. These results suggest that the commercial recombinant tissue plasminogen activator formulation has a narrow margin of safety in nonvitrectomized eyes and that a component of the vehicle is the toxic factor.

Author Affiliations
From the Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami (Fla) School of Medicine. Dr R. Johnson is now in private practice in San Francisco, Calif.

Footnotes
Accepted for publication September 18, 1989.

Read before the annual meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 3, 1989.

Reprint requests to Bascom Palmer Eye Institute, PO Box 016880, Miami, FL 33101 (Dr Olsen).

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