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Experimental Pseudomonas aeruginosa Keratitis From Extended Wear of Soft Contact Lenses
Jörg M. Koch, MD;
Miguel F. Refojo, DSc;
Laila A. Hanninen;
Fee-Lai Leong;
Kenneth R. Kenyon, MD
Arch Ophthalmol. 1990;108(10):1453-1459.
Abstract
• We used a rabbit model to investigate the pathogenesis of soft contact lens—induced bacterial keratitis. Rabbit eyes underwent complete tarsorrhaphy for 7 days either with (group A, n=14) or without (group B, n=13) new sterile soft contact lenses. On day 7, an increase in mean corneal thickness (20.3% in group A and 17.2% in group B) was detected. New or rabbit-worn soft contact lenses were then inoculated with 107 colony-forming units of Pseudomonas aeruginosa or by 0.1 mL of P aeruginosa suspension. On day 9, conjunctival cultures of all eyes yielded P aeruginosa. Corneal infection developed in 11 of 14 eyes wearing new or worn, contaminated soft contact lenses. Bacterial keratitis did not develop in any of the 13 eyes inoculated with P aeruginosa suspension. Light and electron microscopy of infected eyes showed abundant polymorphonuclear neutrophils destroying the epithelium, basement membrane, and stroma. Few bacteria could be detected and only in the deep stroma. Since bacterial suspension alone caused no inflammation, soft contact lens—wear appears crucial to corneal infection in this model.
Author Affiliations
From the Eye Research Institute (Drs Koch, Refojo, and Kenyon, and Mss Hanninen and Leong), and the Department of Ophthalmology (Drs Koch, Refojo, and Kenyon), Harvard Medical School, Boston, Mass. Dr Koch is now at the University of Essen, Eye Clinic, Essen, West Germany.
Footnotes
Accepted for publication June 6, 1990.
Reprint requests to Eye Research Institute, 20 Staniford St, Boston, MA 02114 (Dr Refojo).
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