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Jay S. Pepose, MD, PhD; Gerald Linette, PhD; Steven F. Lee, MD; Scott MacRae, MD

Arch Ophthalmol. 1989;107(7):983-985.

Abstract
• Goldmann tonometer tips were inoculated with 5 x 10⁵ IU of cell-free or cell-associated human immunodeficiency virus type 1 (lymphadenopathy virus type 1 isolate) or 10⁴ plaque-forming units of herpes simplex virus type 1 (McKrae strain) or type 2 (Hicks strain). In an effort to mimic a "worst case" clinical scenario, each respective virus was allowed to air dry on the tonometer tip for 10 minutes. Inoculated tonometers were then (1) not treated, (2) wiped with a disposable (Kimwipe) tissue or sterile gauze; (3) wiped with sterile gauze soaked with 3% hydrogen peroxide; or (4) wiped with a 70% isopropyl alcohol swab. The hydrogen peroxide treatment and the alcohol wipes both completely disinfected the tonometer tips for human immunodeficiency virus type 1 and herpes simplex virus types 1 and 2, whereas wiping with a sterile gauze or tissue was not effective. Wiping the Goldmann tonometer tip with an isopropyl alcohol swab and then allowing the alcohol to evaporate provides a ready and efficient means of inactivating these three enveloped viruses.

Author Affiliations
From the Departments of Ophthalmology (Drs Pepose and Lee) and Pathology (Dr Pepose), Washington University School of Medicine, St Louis, Mo; the Department of Microbiology, Georgetown University School of Medicine, Washington, DC (Dr Linette); and the Department of Ophthalmology, Oregon Health Sciences University, Portland, Ore (Dr MacRae). Dr Pepose is a faculty fellow of the American College of Surgeons (1988-1990).

Footnotes
Accepted for publication March 27, 1989.

Reprint requests to Department of Ophthalmology, Box 8096, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110 (Dr Pepose).

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