This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (174)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Khalil D. Hanna, MD; Yves Pouliquen, MD; George O. Waring III, MD; Michele Savoldelli; John Cotter, MD; Kenneth Morton, MD; Maurice Menasche, PhD

Arch Ophthalmol. 1989;107(6):895-901.

Abstract
• An argon fluoride excimer laser (193 nm) with a moving slit delivery system was used to perform anterior myopic keratomileusis in both eyes of 24 New Zealand white rabbits. Rabbits were killed immediately after ablation and at intervals up to 100 days. By slit-lamp microscopy, four rabbits at day 100 exhibited four clear corneas and four corneas had central, spotty, subepithelial haze. Light and electron microscopy documented corneal healing. In the early stages a transient acellular zone in the anterior stroma appeared over a period of three weeks, followed by an increased number of fibrocytes. In the corneas with opacification, focal areas of 20-µm-thick subepithelial scarring were present. An unexpected finding was transient damage to posterior stromal keratocytes and endothelial cells. The endothelium produced a layer of granular material that migrated anteriorly across Descemet's membrane. Immunochemistry at day 6 showed a marked staining for collagen IV, proteoglycans, fibronectin, and laminin.

Author Affiliations
From the Department of Ophthalmology, Hotel-Dieu Hospital, Paris (Drs Hanna, Pouliquen, Cotter, Morton, and Menasche and Mr Savoldelli); the IBM Paris Scientific Center (Dr Hanna); and the Department of Ophthalmology, Emory University, Atlanta (Drs Hanna and Waring).

Footnotes
Accepted for publication Dec 5, 1988.

Reprint requests to Department of Ophthalmology, Hotel-Dieu Hospital, 1 Place du Parvis Notre-Dame, 75004, Paris, France (Dr Hanna).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

The Effect of Growth Factor Signaling on Keratocytes In Vitro and Its Relationship to the Phases of Stromal Wound Repair
Etheredge et al.
IOVS 2009;50:3128-3136.
ABSTRACT | FULL TEXT  

RANK, RANKL, OPG, and M-CSF Expression in Stromal Cells during Corneal Wound Healing
Wilson et al.
IOVS 2004;45:2201-2211.
ABSTRACT | FULL TEXT  

Keratocyte Density in the Human Cornea After Photorefractive Keratectomy
Erie et al.
Arch Ophthalmol 2003;121:770-776.
ABSTRACT | FULL TEXT  

Normal Human Keratocyte Density and Corneal Thickness Measurement by Using Confocal Microscopy In Vivo
Patel et al.
IOVS 2001;42:333-339.
ABSTRACT | FULL TEXT  

Intact corneal epithelium is essential for the prevention of stromal haze after laser assisted in situ keratomileusis
Nakamura et al.
Br J Ophthalmol 2001;85:209-213.
ABSTRACT | FULL TEXT  

Corneal guttata associated with the corneal dystrophy resulting from a beta ig-h3 R124H mutation
Akimune et al.
Br J Ophthalmol 2000;84:67-71.
ABSTRACT | FULL TEXT  

Analysis of glycosaminoglycans in rabbit cornea after excimer laser keratectomy
Kato et al.
Br J Ophthalmol 1999;83:609-612.
ABSTRACT | FULL TEXT  

In Vivo Confocal Microscopy After Photorefractive Keratectomy in Humans: A Prospective, Long-term Study
Frueh et al.
Arch Ophthalmol 1998;116:1425-1431.
ABSTRACT | FULL TEXT  

Changes in Descemet Membrane and Endothelium After Corneal Epithelial Abrasion Alone and With Photorefractive Keratectomy in Rabbits
Sano et al.
Arch Ophthalmol 1996;114:1105-1108.
ABSTRACT  

Central and Peripheral Endothelial Cell Changes After Excimer Laser Photorefractive Keratectomy for Myopia
Trocme et al.
Arch Ophthalmol 1996;114:925-928.
ABSTRACT  

Human Excimer Laser Keratectomy: Immunohistochemical Analysis of Healing
Anderson et al.
Arch Ophthalmol 1996;114:54-60.
ABSTRACT  

The Corneal Endothelium and Myopic Excimer Laser Photorefractive Keratectomy
Maldonado and Menezo
Arch Ophthalmol 1995;113:697-697.
ABSTRACT  

Prevention of Keratocyte Loss After Corneal Deepithelialization in Rabbits
Nassaralla et al.
Arch Ophthalmol 1995;113:506-511.
ABSTRACT  

The Corneal Endothelium After Myopic Excimer Laser Photorefractive Keratectomy
Carones et al.
Arch Ophthalmol 1994;112:920-924.
ABSTRACT  

Reassembly of Corneal Epithelial Adhesion Structures After Excimer Laser Keratectomy in Humans
Fountain et al.
Arch Ophthalmol 1994;112:967-972.
ABSTRACT  

Keratocyte Loss and Repopulation of Anterior Corneal Stroma After De-epithelialization
Szerenyi et al.
Arch Ophthalmol 1994;112:973-976.
ABSTRACT  

Corneal Wound Healing in Monkeys After Repeated Excimer Laser Photorefractive Keratectomy
Hanna et al.
Arch Ophthalmol 1992;110:1286-1291.
ABSTRACT  

Surface Ultrastructure After Excimer Laser Ablation: Expanding vs Contracting Apertures
Sinbawy et al.
Arch Ophthalmol 1991;109:1531-1533.
ABSTRACT  

Corneal Wound Healing After 193-nm Excimer Laser Keratectomy
Wu et al.
Arch Ophthalmol 1991;109:1426-1432.
ABSTRACT  

Modulation of Corneal Wound Healing After Excimer Laser Keratomileusis Using Topical Mitomycin C and Steroids
Talamo et al.
Arch Ophthalmol 1991;109:1141-1146.
ABSTRACT  

Corneal Epithelial and Stromal Reactions to Excimer Laser Photorefractive Keratectomy: II. Unpredictable Corneal Cicatrization
Cintron
Arch Ophthalmol 1990;108:1540-1541.
ABSTRACT  

Immunofluorescence Study of Corneal Wound Healing After Excimer Laser Anterior Keratectomy in the Monkey Eye
Malley et al.
Arch Ophthalmol 1990;108:1316-1322.
ABSTRACT  

A Comparison of En Face and Tangential Wide-Area Excimer Surface Ablation in the Rabbit
Holme et al.
Arch Ophthalmol 1990;108:876-881.
ABSTRACT  

Wound Healing After Excimer Laser Keratomileusis (Photorefractive Keratectomy) in Monkeys
Fantes et al.
Arch Ophthalmol 1990;108:665-675.
ABSTRACT  

Corneal Healing Following Laser Refractive Keratectomy
Goodman et al.
Arch Ophthalmol 1989;107:1799-1803.
ABSTRACT