A new model of experimental choroidal neovascularization in the rat

E. T. Dobi, C. A. Puliafito and M. Destro
Massachusetts Eye & Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston.

Choroidal neovascularization in rat eyes was induced by krypton laser photocoagulation. Lesions were studied weekly by ophthalmoscopy, fundus photography, and fluorescein angiography. Morphologic correlation was provided by serial sectioning of lesions for light and transmission electron microscopy. In addition, vascular casts were prepared for scanning electron microscopy. Choroidal neovascularization occurred in 25 (60%) of 42 lesions, as evidenced by growth of capillaries through breaks in Bruch's membrane. In addition, 24 (28%) of 86 lesions studied by fluorescein angiography demonstrated leakage. This study provides the most complete angiographic, histologic, and ultrastructural documentation of experimental choroidal neovascularization in the rat. This model may be useful for in vivo studies of choroidal angiogenesis and its modulation via drug therapy.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Dendritic Cells Augment Choroidal Neovascularization
Nakai et al.
IOVS 2008;49:3666-3670.
ABSTRACT | FULL TEXT  

Photodynamic Therapy Induces Caspase-Dependent Apoptosis in Rat CNV Model
Matsubara et al.
IOVS 2007;48:4741-4747.
ABSTRACT | FULL TEXT  

A Novel Imaging Technique for Experimental Choroidal Neovascularization
Campos et al.
IOVS 2006;47:5163-5170.
ABSTRACT | FULL TEXT  

Dose-dependent effect of pitavastatin on VEGF and angiogenesis in a mouse model of choroidal neovascularization.
Zambarakji et al.
IOVS 2006;47:2623-2631.
ABSTRACT | FULL TEXT  

Apolipoprotein E allele-dependent pathogenesis: A model for age-related retinal degeneration
Malek et al.
Proc. Natl. Acad. Sci. USA 2005;102:11900-11905.
ABSTRACT | FULL TEXT  

Nonhuman Primate Models for Diabetic Ocular Neovascularization Using AAV2-Mediated Overexpression of Vascular Endothelial Growth Factor
Lebherz et al.
Diabetes 2005;54:1141-1149.
ABSTRACT | FULL TEXT  

BDNF Reduces the Retinal Toxicity of Verteporfin Photodynamic Therapy
Paskowitz et al.
IOVS 2004;45:4190-4196.
ABSTRACT | FULL TEXT  

Selective Photodynamic Therapy by Targeted Verteporfin Delivery to Experimental Choroidal Neovascularization Mediated by a Homing Peptide to Vascular Endothelial Growth Factor Receptor-2
Renno et al.
Arch Ophthalmol 2004;122:1002-1011.
ABSTRACT | FULL TEXT  

Predilection of the Macular Region to High Incidence of Choroidal Neovascularization After Intense Laser Photocoagulation in the Monkey
Shen et al.
Arch Ophthalmol 2004;122:353-360.
ABSTRACT | FULL TEXT  

The Squirrel Monkey: Characterization of a New-World Primate Model of Experimental Choroidal Neovascularization and Comparison with the Macaque
Criswell et al.
IOVS 2004;45:625-634.
ABSTRACT | FULL TEXT  

Choroidal Neovascularization Is Provided by Bone Marrow Cells
Tomita et al.
Stem Cells 2004;22:21-26.
ABSTRACT | FULL TEXT  

RPE Cells Modulate Subretinal Neovascularization, but Do Not Cause Regression in Mice with Sustained Expression of VEGF
Ida et al.
IOVS 2003;44:5430-5437.
ABSTRACT | FULL TEXT  

Choroidal neovascular membrane inhibition in a laser treated rat model with intraocular sustained release triamcinolone acetonide microimplants
Ciulla et al.
Br. J. Ophthalmol. 2003;87:1032-1037.
ABSTRACT | FULL TEXT  

The critical role of ocular-infiltrating macrophages in the development of choroidal neovascularization
Tsutsumi et al.
J. Leukoc. Biol. 2003;74:25-32.
ABSTRACT | FULL TEXT  

Noninvasive Technique for Monitoring Chorioretinal Temperature during Transpupillary Thermotherapy, with a Thermosensitive Liposome
Miura et al.
IOVS 2003;44:2716-2721.
ABSTRACT | FULL TEXT  

Verteporfin Photodynamic Therapy in the Rat Model of Choroidal Neovascularization: Angiographic and Histologic Characterization
Zacks et al.
IOVS 2002;43:2384-2391.
ABSTRACT | FULL TEXT  

Expression of Pigment Epithelium-Derived Factor in Experimental Choroidal Neovascularization
Renno et al.
IOVS 2002;43:1574-1580.
ABSTRACT | FULL TEXT  

Suppression of Choroidal Neovascularization by Adeno-associated Virus Vector Expressing Angiostatin
Lai et al.
IOVS 2001;42:2401-2407.
ABSTRACT | FULL TEXT  

Intravitreal Triamcinolone Acetonide Inhibits Choroidal Neovascularization in a Laser-Treated Rat Model
Ciulla et al.
Arch Ophthalmol 2001;119:399-404.
ABSTRACT | FULL TEXT  

Intrachoroidal Neovascularization in Transgenic Mice Overexpressing Vascular Endothelial Growth Factor in the Retinal Pigment Epithelium
Schwesinger et al.
Am. J. Pathol. 2001;158:1161-1172.
ABSTRACT | FULL TEXT  

Choroidal Neovascularization in the Rat Induced by Adenovirus Mediated Expression of Vascular Endothelial Growth Factor
Baffi et al.
IOVS 2000;41:3582-3589.
ABSTRACT | FULL TEXT  

Targeted Disruption of the FGF2 Gene Does Not Prevent Choroidal Neovascularization in a Murine Model
Tobe et al.
Am. J. Pathol. 1998;153:1641-1646.
ABSTRACT | FULL TEXT  

Expression of cell adhesion molecules and vascular endothelial growth factor in experimental choroidal neovascularisation in the rat
Shen et al.
Br. J. Ophthalmol. 1998;82:1063-1071.
ABSTRACT | FULL TEXT