This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Jan M. McDonnell, MD; Peter J. McDonnell, MD; Warren C. Stout, MD; W. John Martin, MD

Arch Ophthalmol. 1989;107(11):1631-1634.

Abstract
• The polymerase chain reaction (PCR) is a molecular technique that amplifies specific target DNA sequences in vitro to facilitate identification of DNA. We have applied the PCR to a recurrent infiltrating, well-differentiated squamous carcinoma from the right lower eyelid of a 37-year-old woman. With primers specific for human papillomavirus (HPV) type 16 DNA, PCR yielded a single band of amplified DNA product. The product was positive, with a radiolabeled HPV type 16 probe on dot blot analysis. The presence of HPV type 16 viral DNA in this recurrent squamous carcinoma of the eyelid has implications with regard to the possible origin, treatment, and prognosis of the tumor.

Author Affiliations
From the Doheny Eye Institute (Drs J. McDonnell, P. McDonnell, and Stout), and the Departments of Ophthalmology (Drs J. McDonnell, P. McDonnell, and Stout) and Pathology (Drs J. McDonnell and Martin), University of Southern California School of Medicine, Los Angeles.

Footnotes
Accepted for publication July 20,1989.

Reprint requests to Doheny Eye Institute, 1355 San Pablo St, Los Angeles, CA 90033 (Dr J. M. McDonnell).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Lack of human papillomavirus in pterygium of Chinese patients from Taiwan
Chen et al.
Br J Ophthalmol 2003;87:1046-1048.
FULL TEXT  

Squamous cell carcinoma of the palpebral conjunctiva
MATSUMOTO et al.
Br J Ophthalmol 1997;81:802d-802.
FULL TEXT