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Stephen S. Bylsma; John R. Samples, MD; Ted S. Acott, PhD; E. Michael Van Buskirk, MD

Arch Ophthalmol. 1988;106(4):544-547.

Abstract
• The usefulness of argon laser trabeculoplasty (ALT) in the treatment of some patients with glaucoma has been established; yet, the mechanism of ALT is not understood. The hypothesis that the mechanism involves trabecular cell division was examined. Autoradiographic assessment of tritiated thymidine incorporation into trabecular cell DNA in a human corneoscleral explant organ culture system demonstrated a basal level of DNA replication that increased by 180% in the two days immediately after ALT. When labeling was initiated five days after ALT, DNA replication declined to 70% of the control level. The autoradiographic findings were confirmed using biochemical evaluation of tritiated thymidine incorporation into acid-precipitable macromolecules (DNA) in excised trabecular meshwork. One biologic response of the trabecular meshwork after laser trabeculoplasty is a change in the level of ongoing trabecular cell division.

Author Affiliations
From the Departments of Ophthalmology (Mr Bylsma and Drs Samples, Acott, and Van Buskirk) and Biochemistry (Dr Acott), The Oregon Health Sciences University, Portland.

Footnotes
Accepted for publication Nov 12, 1987.

Reprint requests to Department of Ophthalmology, Mail Code L467, The Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201 (Dr Samples).

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