Experimental retinal detachment. XI. Furosemide-inhibitable fluid absorption across retinal pigment epithelium in vivo
S. Tsuboi and J. E. Pederson
Rhegmatogenous retinal detachments were created in one eye of each of six
cynomolgus monkeys. Total vitrectomy alone was performed in the fellow
eyes. The rate of disappearance of fluorescein sodium injected into the
vitreous cavity was measured with kinetic vitreous fluorophotometry.
Intravitreal 10(-4)M probenecid was used to inhibit active outward
transport of fluorescein. In eyes with retinal detachment, the rate of
fluorescein loss from the vitreous cavity was decreased 44% and 22%
following intravitreal injection of 10(-4) and 10(-5)M furosemide (Lasix),
respectively. Intravitreal 10(-4)M furosemide decreased the rate of
fluorescein loss in fellow eyes by 35%. The rate of fluorescein loss via
the anterior chamber accounted for only 1% to 8% of the total rate of
vitreous fluorescein loss. Thus, it is concluded that intravitreal
absorption across the retinal pigment epithelium.
