 |
|
Transplant of Oral Mucosal Epithelium to Rabbit Ocular Surface Wounds In Vivo
Ilene K. Gipson, PhD;
Harry S. Geggel, MD;
Sandra J. Spurr-Michaud, MS
Arch Ophthalmol. 1986;104(10):1529-1533.
Abstract
• Sheets of epithelium freed of underlying connective tissue and basal lamina with dispase II were obtained from explants of rabbit oral mucosa. Epithelial sheets were then sutured onto abraded (basement membrane intact) or keratectomized corneal-limbal zones or abraded central corneas of anesthetized rabbits. To develop the procedure, allografts to abraded (N=13) and keratectomized wounds (N=3) on the corneal-limbal zone were done. All allografts were retained on the wound bed until the experiment was terminated or rejection occurred. Prior to rejection, eyes were uninflamed and quiet. Allografts on central avascular corneal wounds (N=8) were not maintained. Autografts (N=4) sutured to corneal-limbal abrasion wounds were maintained until animals were killed at the end of the experiment, 22, 26, 60, and 120 days after transplant. Eyes were uninflamed and quiet. A fine vascular bed developed under both allografts and autografts in the peripheral cornea. Grafts maintained some histologic characteristics of oral mucosal epithelium, even after four months. These data indicate that it is feasible to transplant oral mucosal epithelium to corneal-limbal ocular surface wounds and that the grafts will be maintained. Such transplants are, however, not maintained over central, avascular corneal regions.
Author Affiliations
From the Eye Research Institute of Retina Foundation (Dr Gipson and Ms Spurr-Michaud) and the Department of Ophthalmology, Harvard Medical School (Dr Gipson), Boston; and The Mason Clinic, Seattle (Dr Geggel).
Footnotes
Accepted for publication April 18, 1986.
Reprint requests to Eye Research Institute, 20 Staniford St, Boston, MA 02114 (Dr Gipson).
This study was supported in part by research grants R01 EY03306 and F32 EY05626 from the National Institutes of Health, Bethesda, Md.
 CiteULike  Connotea  Delicious  Digg  Facebook  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Persistence of Transplanted Oral Mucosal Epithelial Cells in Human Cornea
Chen et al.
IOVS 2009;50:4660-4668.
ABSTRACT
| FULL TEXT
Ocular Surface Reconstruction Using Autologous Rabbit Oral Mucosal Epithelial Sheets Fabricated Ex Vivo on a Temperature-Responsive Culture Surface
Hayashida et al.
IOVS 2005;46:1632-1639.
ABSTRACT
| FULL TEXT
Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders
Nakamura et al.
Br J Ophthalmol 2004;88:1280-1284.
ABSTRACT
| FULL TEXT
|
