This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (19)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Larry A. Donoso, MD, PhD; Jerry A. Shields, MD; James J. Augsburger, MD; Joy Whitman; Violeta Arbizo, MS

Arch Ophthalmol. 1986;104(1):106-110.

Abstract
• Monoclonal antibody (MAb) MAb8-1H, which identifies an ocular melanoma— associated antigen, and computerassisted cytomorphometric analysis were used to investigate antigenic and cellular heterogeneity in four atypical cases of choroidal or ciliary body melanoma. The presence of one or more well-localized, morphologically distinct areas within each uveal melanoma, suggestive of multiple tumor cell populations, was observed by conventional light microscopy. In one choroidal melanoma with an unusual bilobed configuration, MAb8-1H bound only to the anterior lobe composed of spindle melanoma cells. In the other three cases, MAb8-1H bound selectively to discrete regions within the uveal melanoma corresponding to the well-localized, morphologically distinct areas seen by light microscopy. In each case, a computer was used to calculate the nuclear area of 50 tumor cells corresponding to the areas binding or not binding MAb8-1H. In three of the four cases examined, the differences in mean nuclear area between the discrete populations of tumor cells were statistically significant. Our results suggest that some uveal melanomas are composed of two or more discrete populations of tumor cells with regard to both expression of melanoma-associated antigens and cytomorphologic features.

Author Affiliations
From the Department of Clinical Research of the Retina Service (Dr Donoso and Ms Whitman), the Oncology Service (Drs Shields, Augsburger, and Donoso), and the Department of Pathology (Dr Shields and Ms Arbizo), Wills Eye Hospital and Thomas Jefferson University, Philadelphia.

Footnotes
Accepted for publication July 29, 1985.

Reprint requests to Wills Eye Hospital, Research Division, Ninth and Walnut streets, Philadelphia, PA 19107 (Dr Donoso).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Transformation of Cell Type in Uveal Melanomas: A Quantitative Histologic Analysis
Bechrakis et al.
Arch Ophthalmol 2000;118:1406-1412.
ABSTRACT | FULL TEXT  

An Ocular Melanoma-Associated Antigen: Molecular Characterization
Wang et al.
Arch Ophthalmol 1992;110:399-404.
ABSTRACT  

Antigenic and Cellular Heterogeneity of Primary Uveal Melanomas-Reply
Donoso et al.
Arch Ophthalmol 1986;104:798-799.
ABSTRACT  

Antigenic and Cellular Heterogeneity of Primary Uveal Melanomas
Folberg
Arch Ophthalmol 1986;104:795-798.
ABSTRACT