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Hepatic Metastasis Identified by Hybridoma-Secreted Monoclonal Antibody MAb8-1H

Larry A. Donoso, MD, PhD; Robert Folberg, MD; Richard Naids, MD; James J. Augsburger, MD; Jerry A. Shields, MD; Barbara Atkinson, MD

Arch Ophthalmol. 1985;103(6):799-801.

Abstract
• Monoclonal antibody MAb8-1H, which defines an ocular melanoma-associated antigen retained in fixed tissue, was used to demonstrate metastatic melanoma cells in liver biopsy specimens from five patients with primary choroidal melanoma. In all cases, the monoclonal antibody bound to tumor cells and not to normal hepatocytes. In one case, a welldelineated focus of tumor cells bound to MAb8-1H; in two cases, MAb8-1H appeared to delineate individual tumor cells that were difficult to categorize by conventional staining techniques. The liver biopsy specimens were almost completely replaced by tumor cells in the remaining two cases. This monoclonal antibody may be applicable to the diagnosis of metastatic uveal melanoma and to delineate micrometastasis.

Author Affiliations
From the Department of Clinical Research (Drs Donoso and Naids) and the Oncology Service (Drs Augsburger and Shields), Wills Eye Hospital and Thomas Jefferson University, the Department of Pathology and Laboratory Medicine, University of Pennsylvania (Dr Atkinson), Philadelphia, and the Department of Ophthalmology, University of Iowa Hospital and Clinics, Iowa City (Dr Folberg).

Footnotes
Accepted for publication Feb 8, 1985.

Reprint requests to Wills Eye Hospital, Research Division, Ninth and Walnut streets, Philadelphia, PA 19107 (Dr Donoso).

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