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Immunohistochemical Study

Chi-Chao Chan, MD; David BenEzra, MD, PhD; Su-Ming Hsu, MD; Alan G. Palestine, MD; Robert B. Nussenblatt, MD

Arch Ophthalmol. 1985;103(2):198-202.

Abstract
• Monoclonal antibodies were used to identify cells that form choroidal granulomas and Dalen-Fuchs nodules in sympathetic ophthalmia as well as the granulomas of sarcoid. Immunohistochemical analysis of frozen sections of fresh human tissues obtained after enucleation (sympathetic ophthalmia) and lung biopsy (sarcoid) was performed by the avidin-biotin-peroxidase complex method. The majority of the inflammatory cells within these lesions were identical and were composed mainly of cells originating from bone marrow-derived monocytes (reticuloendothelial system) probably representing immunologically stimulated granulomas. This study suggests possible immune phenomena that may take part in the pathogenesis of sympathetic ophthalmia and provides some insights into the possible mechanism of formation of choroidal granulomas and Dalen-Fuchs nodules in this disease.

Author Affiliations
From the National Eye Institute, Clinical Ophthalmic Immunology Section (Drs Chan, BenEzra, Palestine, and Nussenblatt), and the National Cancer Institute, Division of Cancer Biology and Diagnosis (Dr Hsu), National Institutes of Health (NIH), Bethesda, Md; and the Department of Ophthalmology, Hadassah Hebrew University Hospital, Jerusalem (Dr BenEzra).

Footnotes
Accepted for publication July 28, 1984.

Reprint requests to National Eye Institute, NIH, Bldg 10, Room 10D19, Bethesda, MD 20205 (Dr Chan).

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