 |
|
Endothelial Cells Release a Chemoattractant for Retinal Pigment Epithelial Cells In Vitro
Peter A. Campochiaro, MD;
Bert M. Glaser, MD
Arch Ophthalmol. 1985;103(12):1876-1880.
Abstract
• Though the pathogenesis of choroidal neovascular membranes is uncertain, there is evidence to support a primary dysfunction in the retinal pigment epithelium (RPE). This suggests the possibility that a healthy RPE may provide a physical and/or chemical barrier to subretinal endothelial cell invasion. It has recently been shown that RPE cells in culture produce an inhibitor of neovascularization. Histopathologic evidence suggests that RPE cells tend to surround new blood vessels and contain them. We therefore investigated the possibility that RPE cells are guided toward endothelial cells by chemoattractants. Using a modified Boyden chamber technique, we showed that endothelial cells in culture produce a chemoattractant for RPE cells. The active component is trypsin sensitive, stable at extremes of pH (3 through 10), and nondialyzable (12,000- to 14,000-dalton cutoff). It is partially heat stable but becomes completely heat stable in the presence of 1% sodium dodecyl sulfate. These are all characteristics of the previously described endothelial cell-derived growth factor, suggesting that this mitogen might be the chemoattractant. The ability of RPE cells to be attracted to sites of new blood vessel formation may enhance their potential function as inhibitors of neovascularization.
Author Affiliations
From the Department of Ophthalmology, University of Virginia Medical Center, Charlottesville (Dr Campochiaro), and the Center for Vitreoretinal Research, Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, Baltimore (Dr Glaser).
Footnotes
Accepted for publication June 24, 1985.
Reprint requests to The Johns Hopkins Hospital, 600 N Wolfe St, Maumenee 119, Baltimore, MD 21205 (Dr Glaser).
 CiteULike  Connotea  Delicious  Digg  Facebook  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Debridement of the Pig Retinal Pigment Epithelium In Vivo
Del Priore et al.
Arch Ophthalmol 1995;113:939-944.
ABSTRACT
Serum Is Chemotactic for Retinal-Derived Glial Cells
de Juan et al.
Arch Ophthalmol 1988;106:986-990.
ABSTRACT
Extracellular Modulating Factors and the Control of Intraocular Neovascularization: An Overview
Glaser
Arch Ophthalmol 1988;106:603-607.
ABSTRACT
Ultrastructure of Bruch's Membrane After Krypton Laser Photocoagulation: II. Repair of Bruch's Membrane and the Role of Macrophages
Pollack et al.
Arch Ophthalmol 1986;104:1377-1382.
ABSTRACT
Retinal Pigment Epithelium and Endothelium
Korte
Arch Ophthalmol 1986;104:802-804.
ABSTRACT
A New Look at the Retinal Pigment Epithelium and Its Neighbors
Patz
Arch Ophthalmol 1985;103:1794-1795.
ABSTRACT
|
