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Tissue Culture Studies

Xun Qing, Gu, MD; Glenna L. Fry; Gene F. Lata, PhD; Andrew J. Packer, MD; E. Gary Servais, MD; John C. Hoak, MD; Sohan S. Hayreh, MD, PhD

Arch Ophthalmol. 1985;103(1):111-117.

Abstract
• The proliferative activity of a number of intraocular fluids, bovine retinal extract, and normal serum (from humans and cynomolgus monkeys) was investigated by in vitro tissue culture studies, with the use of tritiated thymidine incorporation by the cultured endothelial cells of human umbilical veins. There was increased tritiated thymidine incorporation by (1) the aqueous, vitreous, and intraocular fluid (IOF) (which filled the eye after lensectomy and vitrectomy) removed from cynomolgus monkey eyes with iris neovascularization or with neovascular glaucoma (NVG) that developed after experimental retinal vein occlusion, (2) by aqueous and vitreous removed from human eyes with NVG or proliferative diabetic retinopathy; (3) by the serum, and (4) by the bovine retinal extract. However, tritiated thymidine incorporation was not increased by the normal aqueous, vitreous, or IOF.

Author Affiliations
From the Departments of Ophthalmology (Drs Gu, Packer, Servais, and Hayreh), Hematology (Dr Hoak and Ms Fry), and Biochemistry (Dr Lata), University of Iowa, Iowa City.

Footnotes
Accepted for publication June 29, 1984.

Reprint requests to Department of Ophthalmology, University Hospitals and Clinics, Iowa City, IA 52242 (Dr Hayreh).

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