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  Vol. 102 No. 8, August 1984 TABLE OF CONTENTS
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Effect of Vitreous on Morphologic Characteristics of Retinal Pigment Epithelial Cells

A New Approach to the Study of Proliferative Vitreoretinopathy

Jesus Vidaurri-Leal, MD; Rebecca Hohman; Bert M. Glaser, MD

Arch Ophthalmol. 1984;102(8):1220-1223.


Abstract



• Normally, human retinal pigment epithelial (RPE) cells in confluent monolayers maintain a hexagonal to oval shape. However, when these monolayers were overlaid with autologous vitreous, the RPE cells elongated and migrated into the vitreous gel as bipolar fibrocytelike cells. We studied the effect of sodium hyaluronate and collagen individually. Retinal pigment epithelial cells overlaid with hyaluronate did not change their morphologic features. In contrast, when RPE monolayers were overlaid with a collagen gel, the cells changed from their normal epithelial characteristics to bipolar fibrocytelike cells that later migrated into the collagen gel. Exposure of RPE cells to vitreous may play a role in the pathogenesis of proliferative vitreoretinopathy.



Author Affiliations



From the Center for Vitreoretinal Research, The Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, Baltimore.


Footnotes



Accepted for publication Jan 13, 1984.

Read in part at the Annual Meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 6, 1983.

Reprint requests to The Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD 21205 (Dr Glaser).

The type I collagen was provided by Ethicon Inc, Somerville, NJ. The type II collagen was provided by Hynda Kleinman, PhD, from the National Institute of Dental Research.

Harry A. Quigley, MD, assisted with the electron microscopic studies. Dennis R. Cain and David G. Emmert provided photographic assistance.



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