 |
|
Experimental Murine Cytomegalovirus Infection of Ocular Structures
James F. Bale, Jr, MD;
Marsha E. O'Neil;
R. Nick Hogan, PhD;
Earl R. Kern, PhD
Arch Ophthalmol. 1984;102(8):1214-1219.
Abstract
• To study experimental cytomegalovirus (CMV) infection of ocular structures, 3-week-old Swiss-Webster mice were inoculated intraperitoneally with a 0% to 20% lethal inoculum of murine CMV (MCMV). Murine cytomegalovirus was recovered from homogenates of eye tissues on days 3, 5, and 7 after inoculation. Peak virus titers, mean of 2.93 ± 0.67 log plaque-forming units of MCMV per gram of ocular tissue occurred on day 5. Urine cytomegalovirus was recovered from explant cultures of eye and optic nerve 14, 21, 60, 90, and 120 days after MCMV inoculation. Murine cytomegalovirus also persisted in intraocular fluids for as long as 90 days. Murine cytomegalovirus infection was confirmed by immunofluorescence antibody staining and transmission electron microscopy. These experiments indicate that MCMV infects the ocular tissues of mice during systemic MCMV infection, and persists in ocular tissues for as long as 120 days after infection. These studies may be directly relevant to ocular disorders that occur during acquired CMV infections of humans, and suggest that ocular tissues may be a site of CMV persistence or latency.
Author Affiliations
From the Division of Pediatric Neurology, the Department of Pediatrics and Neurology, the University of Iowa College of Medicine, Iowa City (Dr Bale, Ms O'Neil); the Laboratory of Molecular Genetics, National Institutes of Neurologic Communicative Disorders and Stroke, the National Institute of Health, Bethseda, Md (Dr Hogan); and the Division of Pediatric Infectious Disease, Department of Pediatrics, University of Utah School of Medicine, Salt Lake City (Dr Kern).
Footnotes
Accepted for publication Jan 4, 1984.
Reprint requests to Department of Pediatrics, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 (Dr Bale).
This study was supported by US Public Health Service Grant No. EY04786-01, National Eye Institute, National Institutes of Health, Bethesda, Md. Dr Bale was supported by a Teacher-Investigator Development Award 7 K07 NS 00805 from the National Institute of Neurological and Communicative Disorders and Stroke.
Paula Sufficool, Kenneth Moore, and Timothy Greiner provided technical assistance with histologie and electron microscopic studies and Jill CofFman provided secretarial assistance.
 CiteULike  Connotea  Delicious  Digg  Facebook  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
In Vivo Imaging of Ocular MCMV Infection
Zinkernagel et al.
IOVS 2010;51:369-374.
ABSTRACT
| FULL TEXT
Ocular Reactivation of MCMV after Immunosuppression of Latently Infected BALB/c Mice
Zhang et al.
IOVS 2005;46:252-258.
ABSTRACT
| FULL TEXT
|
