Selection of therapeutic agents for intraocular proliferative disease. Cell culture evaluation
M. S. Blumenkranz, A. Claflin and A. S. Hajek
A variety of antimetabolites, steroids, and nonsteroidal anti-inflammatory
agents were tested for their ability to inhibit rabbit dermal and
conjunctival fibroblast proliferation in cell culture. Doxorubicin
hydrochloride and fluorouracil produced notable inhibition in
concentrations of less than 1 mg/L. Meclofenamate sodium and indomethacin
produced notable inhibition at concentrations of 11 and 40 mg/L,
respectively. Dexamethasone sodium phosphate and triamcinolone acetonide
produced inhibition at 200 and 150 mg/L, respectively, but paradoxically
increased proliferation almost two-fold at concentrations ranging from 1 to
30 mg/L under identical culture conditions. Methotrexate sodium
demonstrated only limited effectiveness. This assay system may be a useful
approach to drug selection in the treatment of a variety of ocular
proliferative disorders. Fluorouracil may prove to be of significant value
in the treatment of intraocular proliferative disorders.
