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Cell Culture Evaluation

Mark S. Blumenkranz, MD; Alice Claflin, PhD; Anthony S. Hajek, PhD

Arch Ophthalmol. 1984;102(4):598-604.

Abstract
• A variety of antimetabolites, steroids, and nonsteroidal anti-inflammatory agents were tested for their ability to inhibit rabbit dermal and conjunctival fibroblast proliferation in cell culture. Doxorubicin hydrochloride and fluorouracil produced notable inhibition in concentrations of less than 1 mg/L. Meclofenamate sodium and indomethacin produced notable inhibition at concentrations of 11 and 40 mg/L, respectively. Dexamethasone sodium phosphate and triamcinolone acetonide produced inhibition at 200 and 150 mg/L, respectively, but paradoxically increased proliferation almost twofold at concentrations ranging from 1 to 30 mg/L under identical culture conditions. Methotrexate sodium demonstrated only limited effectiveness. This assay system may be a useful approach to drug selection in the treatment of a variety of ocular proliferative disorders. Fluorouracil may prove to be of significant value in the treatment of intraocular proliferative disorders.

Author Affiliations
From the Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine.

Footnotes
Accepted for publication May 16, 1983.

Read before the Annual Spring Meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 1, 1981.

Reprint requests to Bascom Palmer Eye Institute, PO Box 106880, Miami, FL 33101 (Dr Blumenkranz).

This investigation was supported in part by grant 5421-01 from the Veterans Administration Hospital, Miami; grant 1R23 EY 02924-01A1 from the National Eye Institute, National Institutes of Health, Bethesda, Md; and by Research to Prevent Blindness, Inc, New York.

Hoffmann-La Roche Inc, Nutley, NJ, provided the fluorouracil; Merck Sharpe & Dohme, West Point, Pa, provided the indomethacin.

Charlaine Rowlette and Frances Solano provided editorial assistance; Marcilia Halley, Sue Ringer, and Gilbert Fiorentino provided assistance with cell cultures; and Eleut Hernandez and Barbara French prepared the figures.

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