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Vitreous Fluorophotometry Using Carboxyfluorescein and Fluorescein

Norman P. Blair, MD; Carl W. Jones, PhD; Mark M. Rusin, MS

Arch Ophthalmol. 1984;102(12):1810-1814.

Abstract
• Carboxyfluorescein resembles fluorescein in size and spectral characteristics but is much less lipid soluble. Both dyes were used to differentiate between two groups of factors that influence penetration across the blood-retinal barrier: (1) factors that depend on lipid solubility, such as the area of the barrier, and (2) factors independent of lipid solubility, such as opened intercellular junctions or necrotic cells. Vitreous fluorophotometry was performed on normal and diabetic rats after injection of either dye. After the results were adjusted for sources of error, midvitreous-plasma dye ratios for carboxyfluorescein and fluorescein were of the same order of magnitude in normal rats. Ratios for both dyes increased in diabetic rats, and the increases were similar in magnitude. Our results suggest that lipid solubility contributes little to inward transport of these dyes in both the normal and diabetic states.

Author Affiliations
From the Department of Ophthalmology, University of Illinois at Chicago College of Medicine.

Footnotes
Accepted for publication June 26, 1984.

Reprint requests to University of Illinois Eye and Ear Infirmary, 1855 W Taylor St, Chicago, IL 60612 (Dr Blair).

This investigation was supported in part by Public Health Service grants EY 03227 and EY 03106 (Dr Blair), University of Illinois Biological Resources grant 23937, the American Diabetes Association (Dr Jones is a recipient of a career development award from the American Diabetes Association), and Ophthalmic Research Center Core grant EY 1792 from the National Eye Institute, Bethesda, Maryland.

Marlene Heneghan provided secretarial services. Maxine Gere provided editorial assistance. Robert J. Anderson, PhD, performed the statistical analyses. Michael Evans, PhD, performed the HPLC studies. Streptozocin was provided by the Upjohn Company.

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