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Model Studies in Guinea Pigs Immunized Topically With Fluoresceinyl Ovalbumin

Mahin Khatami, PhD; John J. Donnelly, PhD; Thomas John, MD; John H. Rockey, MD, PhD

Arch Ophthalmol. 1984;102(11):1683-1688.

Abstract
• Repeated topical applications of fluoresceinyl ovalbumin (FL-OA) to the conjunctival sac of guinea pigs sensitized them for conjunctival type 1 hypersensitivity reactions and mast cell degranulation. Guinea pigs infected with Ascaris suum, with high titers of circulating anti-A suum IgE and IgG₁ antibody, also produced conjunctival type 1 reactions on topical challenge with A suum antigen. These reactions were no more intense than those of animals topically sensitized and challenged with FL-OA, which in some instances had no detectable serum homocytotropic antibody. Persistently reactive animals that had undergone repeated type 1 conjunctival reactions had histological findings (eg, papillary changes with extensive epithelial eosinophil infiltrates, epithelial thickening or thinning, numerous goblet cells, subepithelial lymphoid cell infiltrates, and new vessel formation) resembling those of human atopic vernal conjunctivitis.

Author Affiliations
From the Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania School of Medicine, Philadelphia.

Footnotes
Accepted for publication March 5, 1984.

Reprint requests to Scheie Eye Institute, Myrin Circle, 51 N 39th St, Philadelphia, PA 19104 (Dr Khatami).

This study was supported by Public Health Service grants EY-03984 and EY-07035, by Fight for Sight, Inc, New York City, by an unrestricted grant from Research to Prevent Blindness, by the Gretel and Eugene Ormandy Teaching and Research Fund, and by the United Nations Development Program/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases.

Patricia E. Taylor and David J. Wolf provided technical assistance.

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