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A Phosphorus Nuclear Magnetic Resonance Study

Jack V. Greiner, DO, PhD; Jonathan H. Lass, MD; Thomas Glonek, PhD

Arch Ophthalmol. 1984;102(11):1676-1677.

Abstract
• The concentrations of corneal phosphatic metabolites in fresh and eye-bank-processed corneas were measured by phosphorus 31 nuclear magnetic resonance of perchloric acid corneal extracts to determine whether metabolic differences exist between these two corneal preparations. Cat corneas were prepared using an eye-bank protocol, including specular microscopic examination and transport to a distant location. Fresh cat corneas were used as controls. The hexose 6-phosphate resonance band, the nucleoside monophosphate band, the phosphodiesters, and adenosine triphosphate of eye-bank-processed corneas were significantly decreased relative to fresh control corneas. Phosphatic metabolites that significantly increased relative to control corneas included inorganic orthophosphate and phosphocreatine. A calculated corneal tissue-energy index was significantly decreased for eye-bank-processed corneas relative to control. This decline demonstrates a compromise in the metabolic energy status of the tissue and is indicative of a diminished ability of the cornea to maintain its complement of high-energy phosphates.

Author Affiliations
From the Department of Pathology (Dr Greiner) and the Nuclear Magnetic Resonance Laboratory (Dr Glonek), Chicago College of Osteopathic Medicine; Department of Ophthalmology, Georgetown University Center for Sight, Washington, DC (Dr Greiner); and Division of Ophthalmology, University Hospitals of Cleveland and Case Western Reserve University (Dr Lass).

Footnotes
Accepted for publication July 19, 1984.

Reprint requests to Chicago College of Osteopathic Medicine, 5200 S Ellis, Chicago, IL 60615 (Dr Greiner).

This study was supported by grant EY-03988 from the National Eye Institute and by the St Anne's Foundation, Cleveland.

The Cleveland Eye Bank provided the use of its facilities and the technical support of Alan Babrosky. Donna G. Peace assisted in the preparation of tissues for NMR spectroscopy.

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