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Norman P. Blair, MD; Ran C. Zeimer, PhD; Mark M. Rusin, MS; José G. Cunha-Vaz, MD, PhD

Arch Ophthalmol. 1983;101(7):1117-1121.

Abstract
• The permeability of the blood-vitreous barrier to fluorescein passing out of the vitreous does not necessarily equal the permeability to fluorescein passing into it. We calculated the ratio between the outward and inward permeability coefficients of the blood-vitreous barrier in eight normal men who ingested 3-g of sodium fluorescein. The calculation was based on the ratio between the serum free fluorescein and the vitreous fluorescein concentrations (as determined by fluorophotometry) when the net transport across the barrier was zero. The outward permeability to fluorescein was 31 ± 18 times (mean ± SD) the inward permeability. To our knowledge, this article provides the first direct evidence for a specialized transport mechanism in humans whereby fluorescein is removed from the vitreous into the blood. The malfunction of this process may be important in human disease. Pharmacologic manipulation of this process may be possible.

Author Affiliations
From the University of Illinois Eye and Ear Infirmary, Chicago (Drs Blair, Zeimer, and Cunha-Vaz and Mr Rusin); and the University of Coimbra (Portugal) (Dr Cunha-Vaz).

Footnotes
Accepted for publication April 15, 1982.

Read in part before the International Symposium on Ocular Fluorophotometry, Paris, April 17, 1982, and at the meeting of the Association for Research in Vision and Ophthalmology, Sarasota, Fla, May 5,1982.

Reprint requests to the University of Illinois Eye and Ear Infirmary, 1855 W Taylor St, Chicago, IL 60612 (Dr Blair).

This investigation was supported by Public Health Service research grants EY03106 and EY03227 (Dr Blair) and Ophthalmic Research Center core grant EY1792 from the National Eye Institute, Bethesda, Md.

Marlene Heneghan provided secretarial assistance and Maxine Gere provided editorial services.

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