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Hepatic Binding Protein Reactivity Toward a Liver-Metastasizing Clone

Larry A. Donoso, MD, PhD; Rose M. Nagy, MS; Rosemary C. McFall; Karen E. Edelberg; David G. Vossler, MD

Arch Ophthalmol. 1983;101(5):787-790.

Abstract
• A clone of B16 malignant melanoma cells with a preference for metastasis to the liver was isolated and characterized. The parent tumor cells (F1) were injected in the tail vein of C57BL/6 mice, and the resultant liver colonizing cells were isolated and then subcultured for two to three weeks. The cells were then reinjected into the next series of mice. After five such passages, a clone (L4) of melanoma cells was obtained that metastasized almost exclusively to the liver. A hepatic binding protein (HBP) was isolated from rabbit liver that agglutinated neuraminidase-treated F1 and L4 malignant melanoma cells. Different agglutination titers found between the parent and liver-metastasizing clone demonstrated differences in cell-surface properties between the parent tumor and the livermetastasizing clone. These results demonstrate that malignant melanoma cells can be selected for preferential liver metastasis and can be recognized and agglutinated by specific HBPs. Metastasis from human uveal malignant melanoma may occur by similar mechanisms.

Author Affiliations
From the Department of Clinical Research, the Retina and Oncology Services (Drs Donoso and Vossler and Ms Edelberg), and the Department of Immunology (Mss Nagy and McFall), Wills Eye Hospital and Thomas Jefferson University, Philadelphia.

Footnotes
Accepted for publication May 3, 1982.

Reprint requests to Wills Eye Hospital, Ninth and Walnut streets, Philadelphia, PA 19107 (Dr Donoso).

This study was supported in part by grant 1R23EY03834-01 (Dr Donoso) from the National Institutes of Health, the Pennsylvania Lions Sight Conservation and Eye Research Foundation, Research to Prevent Blindness Inc, and the American National Red Cross Blood Research Laboratory (M. Wickerhauser, PhD).

The cells lines were provided by I. J. Fidler, PhD, Frederick (Md) Cancer Research Center. James J. Augsburger, MD, Vitaliano B. Bernardino, MD, Robert Folberg, MD, and Jerry A. Shields, MD, provided support for this project.

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ABSTRACT