Centrifugal cytology of ocular fluids
R. D. Stulting, R. C. Leif, J. G. Clarkson and D. Bobbitt
Centrifugal cytology is a new technique for the preparation of ocular
fluids for cytologic examination. It differs from conventional
cytocentrifuge techniques, since fixation is carried out simultaneously
with centrifugation. It avoids air-drying artifacts and can be applied to
small (50 microL) or large (200 mL or more) sample volumes and dilute cell
suspensions, such as might be obtained by anterior chamber paracentesis,
vitreous aspiration, or vitrectomy. The resultant preparation is a
permanent, stained, well-preserved, flattened, homogeneous cell dispersion
suitable for detailed cytologic evaluation.
